A. Safety operation in a PCR lab
- Hazardous chemicals.
- Safety rules for machines operation.
- Reagent preservation and lab security
B. Basic theory of PCR
- DNA structure.
- The function of DNA Polymerase.
- The principle of polymerase chain reaction (PCR).
- Basic requirements and design of PCR lab.
- Potential problems of PCR.
C. Introduction of PCR related reagents:
- Nucleic acid extraction kit.
- IQ2000/IQREAL/IQ Plus /POCKIT Reagent Set.
D. Introduction of PCR related equipment
E. Review of some major diseases
F. Basic theory of viral disease prevention
- Avoid vertical transmission.
- Avoid horizontal transmission.
A. Practice of DNA extraction
B. Practice of PCR operation procedure
- Reaction condition.
- Reagents preparation.
- Reaction Procedure.
C. Practice of gel electrophoresis if needed:
- Agarose gel preparation.
- Electrophoresis.
- Gel staining and data assay.
D. Sampling procedure and sample preservation
- Preservation of samples.
- Preservation of nucleic acid samples.
- Introduction of sample dissecting tools.
- Disinfection procedure of the sample dissecting tools.
- Sampling strategy.
A. Perform the assay by using real samples
- To compare the sensitivity, reproducibility, and to practice more, it is necessary to go through the whole procedure by real samples again.
- Positive standards and negative control are required.
- Requirements of sample.
B. Detail description of PCR diagnostic procedure
- Sampling, sensitivity, and statistical issue.
- False positive or false negative result.
- Reproducibility.
- Lab management.
C. How to integrate the diagnostic results into real practice.
A. Real samples operation: to confirm the operation and process the other diagnostic systems.
B. Discussion.
